Ddr1 assay
WebAssay Format. HEK293 cells transiently expressing NanoLuc-DDR1 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET … WebPathHunter® DDR1 Functional Assay, DiscoverX DiscoverX offers over a decade of experience in generating stable cell lines of unsurpassed quality and leading performance utilizing the proprietary enzyme fragment complementation (EFC) technology against a broad range of target classes (GPCRs, kinases, epigenetics targets (methyltransferases ...
Ddr1 assay
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WebReport: A detailed report including assay conditions and comprehensive evaluation of data as well as raw data for each analysis will be provided. Screening location: Malvern, PA, USA Compound requirements: In brief, 50 to 100 µl of a 10 to 50 mM DMSO stock or 2-3 mg solid material is needed. WebKinase Activity Assays Count on minimal interference from your compound library with luminescent, ADP-based kinase assays. These sensitive and reliable assays are easily scaled to meet your throughput needs. Select from over 370 Kinase Enzyme Systems, including >100 mutant kinases. Learn about Kinase Activity Assays Shop Kinase …
WebGene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay. PrimePCR™ SYBR® Green Assay: DDR1, … WebJan 12, 2024 · DDR1 has five isoforms namely: DDR1a, DDR1b, DDR1c, DDR1d, and DDR1e. Amongst these; DDR1a, DDR1b, and DDR1c are kinase-active containing the KD, and they are full-length functional receptors, whereas DDR1d and DDR1e are kinase-inactive, due to truncation or frame shifts, so they are nonfunctional receptors [ 19 ].
WebDDR1-IN-2 (DDR1 inhibitor 7rh) is a potent, selective, ATP-competitive Discoidin domain receptor 1 (DDR1) inhibitor (IC50: 6.8 nM in cell-free kinase assays). DDR1-IN-2 inhibited the enzymatic activity of DDR1, with IC50 values of 6.8 nM, but were significantly less potent in suppressing the kinase activities of DDR2, Bcr-Abl, and c-Kit. It ... WebDiscovery of a broad spectrum antiproliferative agent with selectivity for DDR1 kinase: cell line-based assay, kinase panel, molecular docking, and toxicity studies Discovery of a broad spectrum antiproliferative agent with selectivity for DDR1 kinase: cell line-based assay, kinase panel, molecular docking, and toxicity studies
WebFeb 9, 2024 · To examine whether the interaction depends on DDR1 kinase activity, we introduced DDR1-K618A for Co-IP assay. The results suggested that the interaction …
WebDDR2 Kinase Assay - Promega Corporation proposed setup for a gaming systemWebAssay Properties Assay Format 384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay ... DDR1 NanoBRET Kinase Assay. Reference Compound IC50 for DDRI 120 100 40 -10 Dasatini -6 -5 Log[Dasatinib] (M) -0.8910 6.991e-009 HILLSLOPE EC50 . Title: View PDF proposed shelf lifeWebMay 12, 2024 · DDR1 promotes cellular proliferation in UM cells The overexpression of DDR1 in UM cells and tissues prompted us to ask whether DDR1 promoted the growth of UM cells. 92.1 and Omm1 cells with... requirement for nursing home administratorWebAdditionally, the results of the gain- and loss-of-function of the DDR1 assay in MC3T3-E1 cells indicated that DDR1 can regulate the osteoblast differentiation through activating autophagy by ... requirement for nursing degreeWebDDR1 Kinase Assay Service Activity-based DDR1 kinase assay for compound screening and profiling via radiometric HotSpot TM kinase assay. Target Overview CAT# DDR1 … requirement for nigerian passport renewalWebTo further investigate the effects of DDR1 on tumor cell growth in vivo, a tumorigenesis assay was performed in BALB/c female mice using 4T1 cells with or without stable DDR1 overexpression. The tumors in the … requirement for new passport in pakistanWebAssay Measures. Receptor Phosphorylation. How it works. Cells were plated in a 96-well plate and incubated for 24 or 48 hours at 37°C/5% CO2. Cells were then stimulated with a control agonist. Following stimulation, … requirement for law school