WebFeb 25, 2024 · The cytosolic fraction was extracted as previously reported ... (MFN2) was used to identify mitochondria, alpha-tubulin was used for detecting cytosol, and histone H3 indicated the nuclear fraction. (C–E) mtDNA was directly detected in the cytosol via qPCR at 2, 4, and 24 h post-TBI. Each animal was normalized to the respective hemisphere’s ... WebReactivity to Lamin A/C nuclear protein and cytosolic α-Tubulin antibodies was used to assess the purity of the nuclear-enriched and cytosolic fractions (Figure 2B). Clearly, the two bands corresponding to the AhR are detected mostly in the nuclear-enriched fractions (89%) with only a very slight cytosolic presence (11%).
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WebDec 13, 2011 · The amounts that we observed in the cytosolic fraction were not evident in the immunofluorescence images (Fig. 3, A and C), … WebDec 13, 2011 · Direct comparison of protein distribution between the nucleus, the cytoplasm, and the plasma membrane is important for understanding cellular processes and, in particular, signal transduction, where cascades generated at the cell surface regulate functions in other cellular compartments, such as the … easy healthy recipes bbc
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WebCell Biolabs’ Nuclear/ Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells. The procedure has been optimized to provide … WebNov 13, 2013 · RNA-seq results from nuclear and cytosolic fractions are compared to polyA+ and total RNA-seq from the same tissue samples. Our results highlight significant advantages of performing RNA-seq on cytosol and nuclear RNAs, as compared to standard RNA-seq protocols. WebThe protein concentrations in the cytosolic and nuclear fractions were determined using the bicinchoninic acid (BCA) method. Redox Status Assay. The redox status of the cortex after induction of CCI was assessed by assaying the level of lipid peroxidation and the levels of antioxidative ability in cytosolic fractions. The antioxidative ability ... easy healthy pumpkin bread recipe