Cap-switching race
WebThe transcriptional start sites of glmS (A) and nagB (B) were determined by RACE experiments (Fig. S2). RACE was performed with a 5’-Full RACE Core Set (Takara Bio Inc., Shiga, Japan),... WebJun 1, 2006 · Capping-RACE: a simple, accurate, and sensitive 5' RACE method for use in prokaryotes. Liu F, Zheng K, Chen HC, Liu ZF. Nucleic Acids Res, 46(21):e129, 01 …
Cap-switching race
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WebCap-switching RACE 第一步以poly (T)作为引物对mRNA3′端克隆。 当新和成cDNA延伸到mRNA5′帽子结构时，加入莫洛尼鼠白血病病毒逆转录酶 (MMLV)在cDNA3′端加入若干胞嘧啶poly (C)。 MMLV所催化的加尾反应 … WebFeb 26, 2024 · In the current study, we developed a 5′ RACE-seq method by coupling a custom template-switching and 5′ RACE assay with targeted nanopore sequencing, to accurately unveil 5′ termini of mRNA targets. ... exploiting the 5′ cap-dependent template-switching mechanism. This methodology results to the production of full-length cDNAs, …
WebAug 10, 2024 · Several different 5′ RACE methods have been developed, and one particularly simple and efficient approach called CapFinder relies on the 5′ cap … WebCap-Switching RACE Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4133 To view this item, select one of the options below: Sign In User Name Password Remember my user name & password. Forgot your user name or password? Can't get past this page? Help with Cookies. Need to Activate? Purchase Short-Term Access
WebMar 8, 2024 · TS Oligo structure. The simple version of a TS Oligo is a DNA oligo sequence that carries 3 riboguanosines (rGrGrG) at its 3' end [ 1 ]. The complementarity between these consecutive rG bases and the 3' … WebIt is essential that you consult the appropriate Material Safety Data Sheets and your institution�s Environmental Health and Safety Office for proper handling of equipment and hazardous materials used in these protocols.
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